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Akif Profile

  Mohd. Akif, Ph.D(Assistant Professor)
  Department of Biochemistry
  School of Life Science
  University of Hyderabad,
  Prof. C.R.Rao Road, Gachibowli,
  Hyderabad, 500046, India
  Tel: Mob: +91-7799350868
  Email: This e-mail address is being protected from spambots. You need JavaScript enabled to view it. ; This e-mail address is being protected from spambots. You need JavaScript enabled to view it.

Education       

PhD – Manipal University, Manipal

Centre for DNA Fingerprinting and Diagnostics (CDFD), Hyderabad (2002-2008)

Awards/Achievements/Position held

  • Assistant Professor, University of Hyderabad, Hyderabad (2011-Present)
  • Postdoctoral Research Fellow, University of Bath, UK (2008-2011)
  • Research Associate, Centre for DNA fingerprinting and Diagnostics, Hyderabad (2007-2008)
  • Awarded JRF/SRF from Council of Scientific  and Industrial Research, New  Delhi, 2001

Research Interest

 The chemical and biological properties of biomolecules are determined precisely by their molecular structure. A variety of methods have been developed and being used to understand these properties of biomolecules.  Among these, macromolecular crystallography is a powerful method which provides a detail chemical structure of biomolecules at the atomic resolution.  The knowledge of X-ray crystal structure of macromolecule (Protein) at the atomic resolution enables unforeseen insight into the functional state of protein and gives an insight into the design of inhibitors against therapeutic targets.

My lab is aimed to determine the structural characterization of macromolecules and macromolecular complexes and understanding of chemical basis of binding of small molecules inhibitor with macromolecules using X-ray crystallography as a primary tool. In addition, Biochemical and Biophysical methods will be used to study several classes of biologically important target proteins. Broadly, current projects focus on proteases/peptidases and proteins involved in host-pathogen interaction.

Selected publications 

  • Yates C.J., Masuyer G, Schwager SL, Akif M, Sturrock ED, Acharya KR. (2014) Molecular and Thermodynamic Mechanisms of the Chloride-dependent Human Angiotensin-I-converting Enzyme (ACE). J Biol Chem. 289; 1798-814
  • Masuyer G, Akif M., Czarny B, Beau F, Schwager SL, Sturrock ED, Isaac RE, Dive V, Acharya KR.(2014). Crystal structures of highly specific phosphinic tripeptide enantiomers in complex with the angiotensin-I converting enzyme. FEBS J. 281:943-56.                       
  • Akif M, Masuyer G, Bingham R.J, Sturrock ED, Isaac R.E., Acharya K.R. (2012) Structural basis of peptide recognition by the angiotensin-1 converting enzyme homologue AnCE from Drosophila melanogaster FEBS J. 279: 4525-4534
  • Akif M, Masuyer G., Schwager S.L., Bhuyan B.J., Mugesh G., Isaac R.E., Sturrock E.D, Acharya K.R. (2011) Structural characterization of angiotensin I-converting enzyme in complex with a selenium analogue of captopril. FEBS J. 278: 3644-3650.
  • Akif M., Schwager S.L., Anthony C.S., Czarny B., Beau F., Dive V., Sturrock E.D and Acharya K.R (2011) Novel mechanism of inhibition of human Angiotensin-I converting enzyme (ACE) by a highly specific phosphinic tripeptide. Biochemical J. 436: 53-59.
  • Akif M., Ntai I., Sturrock E.D., Isaac R.E., Bachmann B.O., and Acharya K.R. (2010) Crystal structure of a phosphonotripeptide K-26 in complex with angiotensin converting enzyme homologue (AnCE) from Drosophila melanogaster. Biochem Biophys Res Commun.  398(3):532-536.
  • Akif M., Georgiadis D., Mahajan A., Dive V., Sturrock E.D., Isaac R.E., and Acharya K.R. (2010)  High-resolution crystal structures of Drosophila melanogaster angiotensin-converting enzyme in complex with novel inhibitors and antihypertensive drugs.  J. Mol. Biol. 400(3):502-517.
  • Kumar P., Joshi D.C., Akif M., Akhter Y., Hasnain S.E., and Mande S.C. (2010) Mapping conformational transitions in cyclic AMP receptor protein: crystal structure and normal-mode analysis of Mycobacterium tuberculosis apo-cAMP receptor protein. Biophysical J. 98(2):305-314
  • Akif M., Khare G., Tyagi A.K., Mande S.C., and Sardesai A.A. (2008) Functional studies of multiple thioredoxins from Mycobacterium tuberculosis. J Bacteriol. 190(21):7087-7095.
  • Akif M., Suhre K., Verma C., and Mande S.C. (2005) Conformational flexibility of Mycobacterium tuberculosis thioredoxin reductase: crystal structure and normal-mode analysis. Acta Crystallogr D Biol Crystallogr. 61:1603-1611.